Moyano-Cires Ivanoff, Paula VivianaGarcía Lobo, JimenaGarcía Sánchez, José ManuelPelayo AMuñoz Calero, PFrejo Moya, María TeresaAnadón Baselga, María JoséLobo Alonso, MargaritaPino Sans, Javier Del2025-01-212025-01-212020Moyano, P., García, J., García, J. M., Pelayo, A., Muñoz-Calero, P., Frejo, M. T., Anadon, M. J., Lobo, M., & Del Pino, J. (2020). Chlorpyrifos-induced cell proliferation in human breast cancer cell lines differentially mediated by estrogen and aryl hydrocarbon receptors and KIAA1363 enzyme after 24 h and 14 days exposure. Chemosphere, 251. https://doi.org/10.1016/J.CHEMOSPHERE.2020.1264260045-653510.1016/j.chemosphere.2020.126426https://hdl.handle.net/20.500.14352/115463CRediT authorship contribution statement: Paula Moyano: Methodology, Investigation, Writing - original draft, Writing - review & editing. Jimena García: Investigation, Validation. Jose Manuel García: Validation, Writing - review & editing. Adela Pelayo: Resources, Supervision. Pilar Munoz- ~ Calero: Funding acquisition, Visualization. María Teresa Frejo: Formal analysis, Writing - review & editing. Maria Jose Anadon: Data curation, Writing - review & editing. Margarita Lobo: Formal analysis, Data curation. Javier Del Pino: Conceptualization, Methodology, Project administration, Supervision, Writing - review & editing.Organophosphate biocide chlorpyrifos (CPF) is involved with breast cancer. However, the mechanisms remain unknown. CPF increases cell division in MCF-7 cells, by estrogen receptor alpha (ERa) activation, although it is a weak ERa agonist, suggesting other mechanisms should be involved. Aromatic hydrocarbon receptor (AhR) activation increases cell division in human breast cancer cells, and CPF strongly activates it. Finally, the KIAA1363 enzyme, which is regulated by CPF, is overexpressed in cancer cells. Accordingly, we hypothesized that CPF or its metabolite chlorpyrifos-oxon (CPFO) could induce cell viability promotion in MCF-7 and MDA-MB-231 cell lines, through mechanisms related to ERa, AhR, and KIAA1363, after 24 h and 14 days treatment. Results show that, after acute and long-term treatment, CPF and CPFO alter differently KIAA1363, AhR, ER and cytochrome P450 isoenzyme 1A1 (CYP1A1) expression. In addition, they induced cell proliferation through ERa activation after 24 h exposure in MCF-7 cells and through KIAA1363 overexpression and AhR activation in MCF-7 and MDA-MB-231 cells after acute and long-term treatment. The results obtained in this work provide new information relative to the mechanisms involved in the CPF toxic effects that could lead to breast cancer disease.engjournal article1879-1298https://doi.org/10.1016/j.chemosphere.2020.12642632171938restricted access615.9MCF-7MDA-MB-231ChlorpyrifosERAhRCYP1A1KIAA163Toxicología (Medicina)3214 Toxicología