Petrus, Paul et al.Rydén M.Maldonado Bautista, EstelaMartínez Álvarez, María Concepción2024-02-142024-02-142017cite Petrus P, Mejhert N, Corrales P, Lecoutre S, Li Q, Maldonado E, Kulyté A, Lopez Y, Campbell M, Acosta JR, Laurencikiene J, Douagi I, Gao H, Martínez-Álvarez C, Hedén P, Spalding KL, Vidal-Puig A, Medina-Gomez G, Arner P, Rydén M. Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue. Cell Rep. 2018 Oct 16;25(3):551-560.e5.2211-124710.1016/j.celrep.2018.09.069https://hdl.handle.net/20.500.14352/101438White adipose tissue (WAT) mass is determined by adipocyte size and number. While adipocytes are continuously turned over, the mechanisms controlling fat cell number in WAT upon weight changes are unclear. Herein, prospective studies of human subcutaneous WAT demonstrate that weight gain increases both adipocyte size and number, but the latter remains unaltered after weight loss. Transcriptome analyses associate changes in adipocyte number with the expression of 79 genes. This gene set is enriched for growth factors, out of which one, transforming growth factor-b3 (TGFb3), stimulates adipocyte progenitor proliferation, resulting in a higher number of cells undergoing differentiation in vitro. The relevance of these observations was corroborated in vivo where Tgfb3+/ mice, in comparison with wild-type littermates, display lower subcutaneous adipocyte progenitor proliferation, WAT hypertrophy, and glucose intolerance. TGFb3 is therefore a regulator of subcutaneous adipocyte number and may link WAT morphology to glucose metabolism.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://www.sciencedirect.com/science/article/pii/S2211124718315298?via%3Dihubopen access611611.013White adipose tissueTGFb3Tgfb3+/- miceGlucose intoleranceAdipocyteAdipose hypertrophyCiencias Biomédicas24 Ciencias de la Vida32 Ciencias Médicas