Pérez González, AnaMarconi, M.Cobo Simón, I.Méndez Cea, BelénPerdiguero Jiménez, PedroLinacero De La Fuente, M. RosarioLinares, J. C.Gallego Rodríguez, Francisco Javier2025-12-162025-12-162018-07-10A Pérez-González, M Marconi, I Cobo-Simón, B Méndez-Cea, P Perdiguero, R Linacero, J C Linares, F J Gallego, Abies pinsapo Boiss. Transcriptome Sequencing and Molecular Marker Detection: A Novel Genetic Resources for a Relict Mediterranean Fir, Forest Science, Volume 64, Issue 6, December 2018, Pages 609–6170015-749X10.1093/forsci/fxy022https://hdl.handle.net/20.500.14352/129135I. Cobo is a recipient of a predoctoral fellowship (Ministerio de Economía y Competitividad, BES-2014-070379). J. C. Linares acknowledges financial support by CGL2013-48843-C2-2-R (Ministerio de Economía y Competitividad) and TRANSHABITAT (European Union FEDER 0087). P. Perdiguero acknowledges funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007–2013) under REA grant agreement n° (PIEF-GA-2013-627761).Predicting the coping responses of tree species to climate change is, in many cases, limited by a lack of fundamental genetic insights, such as detailed knowledge of their genomes. Relict tree species may serve as models to assess the general mechanisms and patterns of forest tree responses to the changing climate and to explore the adaptive capacity of trees. Here, we focus on Abies pinsapo, the southernmost European fir, whose marginal populations are prone to extinction. To discover new insights into the genetics of this species, Illumina de novo transcriptome sequencing was performed. We obtained a 24 Gb transcriptome dataset, and the assembly identified 97,768 unigenes with an average length of 1,100 nucleotides. A large number of molecular markers, 100,949 single nucleotide polymorphisms (SNPs), and 5,663 microsatellites (SSRs) were detected, and a gene-associated SNP validation pipeline was developed. The estimated accuracy of the SNP prediction was 55 percent. The novel reference transcriptome and the large number of molecular markers specific to Abies pinsapo reported in this study represent valuable resources for further genetic analyses and conservation guidelines for this endangered tree species and other Abies species.engjournal article1938-3738https://doi.org/10.1093/forsci/fxy022https://academic.oup.com/forestscience/article-abstract/64/6/609/5051094?redirectedFrom=fulltext&login=truerestricted access582.475581.526.42630*2581.15575.17551.588.7TranscriptomeSNPsSSRsAbiesPopulation geneticsBotánica (Biología)Genética2417 Biología Vegetal (Botánica)3106 Ciencia Forestal3106.08 Silvicultura2417.14 Genética Vegetal2409.03 Genética de Poblaciones2502.03 Bioclimatología