Paniagua Herranz, LucíaGómez Villafuertes, María Rosade Agustín Durán, DavidGascón Jiménez, SergioPérez Sen, RaquelGarcía Delicado, EsmerildaMiras Portugal, María TeresaOrtega De La O, FelipeKursad Turksen2025-12-092025-12-092020Paniagua-Herranz L, Gómez-Villafuertes R, Agustín-Durán D, Gascón S, Pérez-Sen R, Delicado EG, Miras-Portugal MT, Ortega F (2020). Time-Lapse Video Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro. In: Turksen, K. (eds) Imaging and Tracking Stem Cells. Methods in Molecular Biology, vol 2150. Humana, New York, NY. https://doi.org/10.1007/7651_2019_2191064-374510.1007/7651_2019_219https://hdl.handle.net/20.500.14352/128631A comprehensive understanding of the mechanisms controlling the behavior of cell populations with regenerative potential is the first step to design effective therapeutic strategies for many diseases. However, a precise description of the biological events involved, such as proliferation, differentiation, cell fate decisions, migration, or viability, may be hampered by the classical use of experiments based on end-point analysis. By contrast, live imaging and single cell tracking provides researchers with an accurate readout of these features in cells throughout an experiment. Here, we describe a protocol to apply time-lapse video microscopy and post-processing of the data to study critical aspects of the biology and the lineage progression of multiple neural populations.engbook parthttps://link.springer.com/protocol/10.1007/7651_2019_21931020634https://link.springer.com/protocol/10.1007/7651_2019_219https://www.webofscience.com/wos/alldb/full-record/WOS:000680871700015metadata only access57761576Live imagingTime-lapse video microscopySingle-cell trackingNeural cellsLineage progressionLlineage treeNeurociencias (Biológicas)Bioquímica (Biología)Biología molecular (Biología)Biología celular (Biología)Farmacología (Medicina)2490 Neurociencias2403 Bioquímica2302.22 Farmacología Molecular2415 Biología Molecular