Ojeda Fernández, IreneMoreno Guzmán, MaríaGonzález Cortés, AraceliYáñez Sedeño, PalomaPingarrón Carrazón, José Manuel2023-06-202023-06-202013(Online )1521-410910.1002/elan.201300269https://hdl.handle.net/20.500.14352/42743Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.engjournal articlehttp://onlinelibrary.wiley.com/doi/10.1002/elan.201300269/fullopen access543CeruloplasminElectrochemical magnetic immunosensorHuman serumQuímica analítica (Química)2301 Química Analítica