Molecular organization of small plasmids bearing blaTEM-1 and conferring resistance to β-lactams in Haemophilus influenzae

Søndergaard, AnnetteSan Millan, AlvaroSantos López, AlfonsoNielsen, Signe MGonzález Zorn, BrunoNørskov-Lauritsen, Niels2023-06-202023-06-202012-091098-659610.1128/AAC.00408-12https://hdl.handle.net/20.500.14352/45168TEM-1 is the dominant β-lactamase of Haemophilus influenzae and can be located on small plasmids. Three distinct plasmids with sizes from 4,304 to 5,646 nucleotides (nt) were characterized: pA1606, pA1209, and pPN223. In addition to TEM-1 and a replication enzyme of the Rep 3 superfamily, pA1606 carries a Tn3 resolvase gene and pA1606 and pA1209 carry an open reading frame (ORF) similar to a plasmid recombination enzyme gene described in Gram-positive bacteria. The plasmids transformed strain Rd to the ampicillin-resistant phenotype.engMolecular organization of small plasmids bearing blaTEM-1 and conferring resistance to β-lactams in Haemophilus influenzaejournal articlehttp://aac.asm.org/content/56/9/4958.fullrestricted accessVeterinaria3109 Ciencias Veterinarias