Pujol-Carrion, NuriaPavón Verges, MónicaArroyo Nombela, Francisco JavierTorre-Ruiz, Maria Angeles de la2026-01-132026-01-132021-04Pujol-Carrion, N., Pavón-Vergés, M., Arroyo, J., de la Torre-Ruiz, MA.The MAPK Slt2/Mpk1 plays a role in iron homeostasis through direct regulation of the transcription factor Aft1. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. Volume 1868, Issue 5, April 2021, 118974.10.1016/j.bbamcr.2021.118974https://hdl.handle.net/20.500.14352/130109Iron is an essential element for life. Cells develop mechanisms to tightly regulate its homeostasis, in order to avoid abnormal accumulation and the consequent cell toxicity. In budding yeast, the high affinity iron regulon is under the control of the transcription factor Aft1. We present evidence demonstrating that the MAPK Slt2 of the cell wall integrity pathway (CWI), phosphorylates and negatively regulates Aft1 activity upon the iron depletion signal, both in fermentative or respiratory conditions. The lack of Slt2 provokes Aft1 dysfunction leading to a shorter chronological life span. The signal of iron scarcity is not transmitted to Slt2 through other signalling pathways such as TOR1, PKA, SNF1 or TOR2/YPK1. The observation that Slt2 physically binds Aft1 rather suggests a direct regulation.engjournal articlehttps://doi.org/10.1016/j.bbamcr.2021.118974open access579Iron homeostasisMAPKSlt2Aft1Chronological life spanMicrobiología (Farmacia)2414 Microbiología2415.01 Biología Molecular de Microorganismos