Deghima, AmiroucheRighi, NadjatRosales Conrado, NoeliaLeón González, María Eugenia DeBaali, FaizaGómez Mejía, EstherMadrid Albarrán, María YolandaBedjou, FatihaSpringer2024-01-112024-01-112020Deghima, A; Righi, N; Rosales-Conrado, N; Leon-Gonzalez, ME; Baali, F; Gomez-Mejia, E; Madrid, Y Bedjou, F, Valorisation of the Green Waste Parts from Large-Leaved Buttercup (Ranunculus macrophyllus Desf.): Phenolic Profile and Health Promoting Effects Study. 2020, 12(8):4307-43181877-264110.1007/s12649-020-01310-zhttps://hdl.handle.net/20.500.14352/92473Due to the extensive use of Ranunculus macrophyllus Desf. roots for medicinal purposes, most of the leafy green parts are just wasted. The aim of this work is to valorize the leafy green parts and promote their application in different modern industries. Methods For this purpose, we studied the phenolic profile of R. macrophyllus Desf. (RM-B) using chromatographic and spectrophotometric methods and we tested the in-vitro antioxidant activity and the in-vivo effect of RM-B on plasma and liver antioxidant statuts. Results RM-B contained high amounts of polyphenols (675 mg GAE/100 g dry weigh dw) and flavonoids (105 mg QE/100 g dw). In-vitro, RM-B exhibited promising radical scavenging activity against 2,2′-azino-bis(3-éthylbenzothiazoline-6-sulphonique) (ABTS+·) (IC50: 247 µg/mL), hydrogen peroxide radicals (IC50: 626 µg/mL) and inhibited oxidative red blood cells hemolysis (IC50: 120 µg/mL), RM-B also showed strong reducing power (982 µM FeSO4/mg extract). In-vivo, RM-B improved the radical scavenging ability and reducing power of plasma and enhanced liver antioxidant status by increasing catalase and reduced glutathione levels and decreasing malondialdhyde levels without altering the key serum biochemical parameters reflecting liver and kidney functions. Polyphenols identified using capillary LC-DAD and LC–MS/MS analyses like hesperidin (131.2 mg/100 g dw), rutin (29.0 mg/100 g dw) and p-coumaric acid (5.8 mg/100 g dw), may be responsible for the health promoting effects of RM-B. Conclusion We may conclude that R. macrophyllus Desf. is a good source of beneficial polyphenols with strong antioxidant, anti-hemolytic and health-promoting effects, which promotes its use in pharmaceutical, medicinal and nutraceutical industries.engjournal article1877-265Xhttps://doi.org/10.1007/s12649-020-01310-zrestricted access543Raw materialMedicinal plants wasteAntioxidantPolyphenolsCatalaseLipid peroxidationQuímica2301 Química Analítica