Rodrigo Martín-Romo, José AugustoAlieva, Tatiana Krasheninnikova2023-06-192023-06-192014-10-01[1] P. Ferraro, A. Wax, and Z. Zalevsky, eds., Coherent Light Microscopy: Imaging and Quantitative Phase Analysis (Springer, 2011). [2] B. Redding, M. A.Choma, and H. Cao, “Speckle-free laser imaging using random laser illumination,” Nat Photon 6, 355–359 (2012). [3] B. Kemper, S. Sturwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, “Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces,” Opt. Lasers Eng. 46, 499 – 507 (2008). [4] T. Kim, R. Zhou, M. Mir, S. D. Babacan, P. S. Carney, L. L. Goddard, and G. Popescu, “White-light diffraction tomography of unlabelled live cells,” Nat Photon pp. 256–263 (2014). [5] D. Paganin and K. A. Nugent, “Noninterferometric phase imaging with partially coherent light,” Phys. Rev. Lett. 80, 2586–2589 (1998). [6] K. G. Phillips, C. R. Velasco, J. Li, A. Kolatkar, M. Luttgen, K. Bethel, B. Duggan, P. Kuhn, and O. Mc-Carty, “Optical quantification of cellular mass, volume and density of circulating tumor cells identified in an ovarian cancer patient,” Frontiers in Oncology 2 (2012). [7] J. A. Rodrigo and T. Alieva, “Rapid quantitative phase imaging for partially coherent light microscopy,” Opt. Express 22, 13472–13483 (2014). [8] E. C. Samson and C. M. Blanca, “Dynamic contrast enhancement in widefield microscopy using projectorgenerated illumination patterns,” New Journal of Physics 9, 363 (2007). [9] J. N. Stirman, M. M. Crane, S. J. Husson, A. Gottschalk, and H. Lu, “A multispectral optical illumination system with precise spatiotemporal control for the manipulation of optogenetic reagents,” Nat. Protocols 7, 207–220 (2012). [10] D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination Super-resolution and optical sectioning microscopy,” Sci. Rep. 3 (2013). [11] M. Born and E. Wolf, Principles of Optics (Cambridge University Press, UK, 2006). [12] A. C. Schell, “The multiple plate antenna,” Ph.D. thesis, Massachusetts Institute of Technology (1961). [13] J. W. Goodman, Statistical Optics (Wiley&Sons, NY, 2000). [14] M. A. Herráez, D. R. Burton, M. J. Lalor, and M. A. Gdeisat, “Fast two-dimensional phase-unwrapping algorithm based on sorting by reliability following a noncontinuous path,” Appl. Opt. 41, 7437–7444 (2002). [15] T. J. McIntyre, C. Maurer, S. Fassl, S. Khan, S. Bernet, and M. Ritsch-Marte, “Quantitative SLM-based differential interference contrast imaging,” Opt. Express 18, 14063–14078 (2010).0146-959210.1364/OL.39.005634https://hdl.handle.net/20.500.14352/34674© 2014 Optical Society of America. Spanish Ministerio de Economia y Competitividad is acknowledged for funding the project TEC2011-23629.Partially coherent illumination provides significant advantages such as speckle-free imaging and enhanced optical sectioning in optical microscopy. The knowledge of the spatial and temporal coherence is crucial to obtain accurate quantitative phase imaging (QPI) of specimens such as live cells, micrometer-sized particles, etc. In this Letter, we propose a novel technique for illumination coherence engineering. It is based on a DMD projector providing fast switchable both multi-wavelength and spatial coherence design. Its performance is experimentally demonstrated for QPI with different spatial coherence states.engjournal articlehttp://dx.doi.org/10.1364/OL.39.005634https://www.osapublishing.orgopen access535MicroscopyLightÓptica (Física)2209.19 Óptica Física