Montero Herradón, SaraGarcía-Ceca Hernández, José JavierZapata González, Agustín Gregorio2024-01-232024-01-232017Montero-Herradón, S., García-Ceca, J., & Zapata, A. G. (2017). EphB receptors, mainly EphB3, contribute to the proper development of cortical thymic epithelial cells. Organogenesis, 13(4), 192–211. https://doi.org/10.1080/15476278.2017.13893681547-627810.1080/15476278.2017.1389368https://hdl.handle.net/20.500.14352/94811This work was supported by the Spanish Ministry of Economy and Competitiveness under grants BFU2013-41112-R and Cell Therapy Network (RD12/0019/0007 and RD16/0011/0002).EphB and their ligands ephrin-B are an important family of protein tyrosine kinase receptors involved in thymocyte-thymic epithelial cell interactions known to be key for the maturation of both thymic cell components. In the present study, we have analyzed the maturation of cortical thymic epithelium in EphB-deficient thymuses evaluating the relative relevance of EphB2 and EphB3 in the process. Results support a relationship between the epithelial hypocellularity of mutant thymuses and altered development of thymocytes, lower proportions of cycling thymic epithelial cells and increased epithelial cell apoptosis. Together, these factors induce delayed development of mutant cortical TECs, defined by the expression of different cell markers, i.e. Ly51, CD205, MHCII, CD40 and b5t. Furthermore, although both EphB2 and EphB3 are necessary for cortical thymic epithelial maturation, the relevance of EphB3 is greater since EphB3¡/¡ thymic cortex exhibits a more severe phenotype than that of EphB2-deficient thymuses.engjournal articlehttps://doi.org/10.1080/15476278.2017.1389368https://www.tandfonline.com/action/downloadSupplement?doi=10.1080%2F15476278.2017.1389368&file=kogg_a_1389368_sm2795.ziprestricted access577.2DevelopmentEphBThymusThymic epithelial cellsThymic cortexThymic epithelial cell survivalThymic epithelial cell cycleBiología molecular (Biología)2415 Biología Molecular