Quinchia, JenniferBlázquez-García, MarinaTorrente Rodríguez, Rebeca MagnoliaRuiz Valdepeñas Montiel, VíctorSerafín González-Carrato, VerónicaRejas-González, RaquelMontero-Calle, AnaOrozco, JahirPingarrón Carrazón, José ManuelBarderas Manchado, RodrigoCampuzano Ruiz, Susana2024-07-052024-07-052024-01Jennifer Quinchia, Marina Blázquez-García, Rebeca M. Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Verónica Serafín, Raquel Rejas-González, Ana Montero-Calle, Jahir Orozco, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano, Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion, Talanta, Volume 267, 2024, 125155, ISSN 0039-9140,0039-914010.1016/j.talanta.2023.125155https://hdl.handle.net/20.500.14352/105710Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin and mucin domain 1 (TIM-1) protein as a diagnostic/prognostic biomarker of high-prevalence breast and lung cancers by using an amperometric disposable magnetic microparticles-assisted immunoplatform. The developed method integrates the inherent advantages of carboxylic acid-functionalized magnetic beads (HOOC-MBs) as pre-concentrator support and the amperometric transduction at screen-printed carbon electrodes (SPCEs). The immunoplatform involves a sandwich-type immunoassay assembled on HOOC-MBs through the specific capture/labeling of TIM-1 using capture antibodies and horseradish peroxidase (HRP)-conjugated biotinylated detection antibodies as biorecognition elements. The magnetic immunoconjugates were confined onto the working electrode (WE) surface of the SPCEs for amperometric detection using the hydroquinone/hydrogen peroxide/HRP (HQ/H2O2/HRP) redox system. The method allows the selective detection of TIM-1 protein over the 87–7500 pg mL−1 concentration range in only 45 min, with a limit of detection of 26 pg mL−1. The developed bioplatform was successfully applied to the analysis of breast and lung cancer cell extracts, providing the first quantitative results of the target glycoprotein in these types of samples.engAttribution-NonCommercial-NoDerivatives 4.0 Internationaljournal articlehttps://doi.org/10.1016/j.talanta.2023.125155https://www.sciencedirect.com/science/article/pii/S0039914023009062?via%3Dihubopen access543577.1TIM-1Amperometric detectionImmunoplatformScreen-printed carbon electrodeMagnetic particlesBreast and lung cancerCiencias2301 Química Analítica2302 Bioquímica