Rodríguez Rodríguez, ElenaBeltrán De Miguel, Beatriz TeresaSamaniego Aguilar, Kerly X.Sánchez Prieto, MilagrosEstévez Santiago, RocíoOlmedilla Alonso, Begoña2023-06-172023-06-172020-06-03Rodríguez Rodríguez, E., Beltrán De Miguel, B. T., Samaniego Aguilar, K. X. et al. «Extraction and Analysis by HPLC-DAD of Carotenoids in Human Faeces from Spanish Adults». Antioxidants, vol. 9, n.o 6, junio de 2020, p. 484. DOI.org (Crossref), https://doi.org/10.3390/antiox9060484.2076-392110.3390/antiox9060484https://hdl.handle.net/20.500.14352/8309Carotenoids are bioactive compounds with widely accepted health benefits. Their quantification in human faeces can be a useful non-invasive approach to assess their bioavailability. Identification and quantification of major dietary carotenoids in human faeces was the aim of the present study. Faeces and dietary intake were obtained from 101 healthy adults (45–65 years). Carotenoid concentrations were determined by HPLC in faeces and by 3-day food records in dietary intake. Carotenoids quantified in faeces (μg/g dry weight, median) were: β-carotene (39.5), lycopene (20), lutein (17.5), phytoene (11.4), zeaxanthin (6.3), β-cryptoxanthin (4.5), phytofluene (2.9). α-carotene (5.3) and violaxanthin were found 75.5% and 7.1% of the faeces. The carotenoids found in the highest concentrations corresponded to the ones consumed in the greatest amounts (μg/d): lycopene (13,146), phytoene (2697), β-carotene (1812), lutein+zeaxanthin (1148). Carotenoid concentration in faeces and in dietary intake showed correlation for the total non-provitamin A carotenoids (r = 0.302; p = 0.003), phytoene (r = 0.339; p = 0.001), phytofluene (r = 0.279; p = 0.005), lycopene (0.223; p = 0.027), lutein+zeaxanthin (r = 0.291; p = 0.04) and β-cryptoxanthin (r = 0.323; p = 0.001). A high proportion of dietary carotenoids, especially those with provitamin A activity and some of their isomers, reach the large intestine, suggesting a low bioavailability of their intact forms.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://doi.org/10.3390/antiox9060484https://www.mdpi.com/2076-3921/9/6/484open accessCarotenesXanthophyllsDietStoolBioavailabilityMiddle-age peopleBromatología (Farmacia)Dietética y nutrición (Farmacia)