Díaz Mendoza, María MercedesBlanca Velasco-Arroyo, BlancaGonzález-Melendi, PabloMartínez, ManuelDíaz, Isabel2024-01-292024-01-292014Mercedes Díaz-Mendoza, Blanca Velasco-Arroyo, Pablo González-Melendi, Manuel Martínez, Isabel Díaz, C1A cysteine protease–cystatin interactions in leaf senescence, Journal of Experimental Botany, Volume 65, Issue 14, July 2014, Pages 3825–3833, https://doi.org/10.1093/jxb/eru0430022-095710.1093/jxb/eru043https://hdl.handle.net/20.500.14352/95900Senescence-associated proteolysis in plants is a crucial process to relocalize nutrients from leaves to growing or storage tissues. The massive net degradation of proteins involves broad metabolic networks, different subcellular compartments, and several types of proteases and regulators. C1A cysteine proteases, grouped as cathepsin L-, B-, H-, and F-like according to their gene structures and phylogenetic relationships, are the most abundant enzymes responsible for the proteolytic activity during leaf senescence. Besides, cystatins as specific modulators of C1A peptidase activities exert a complex regulatory role in this physiological process. This overview article covers the most recent information on C1A proteases in leaf senescence in different plant species. Particularly, it is focussed on barley, as the unique species where the whole gene family members of C1A cysteine proteases and cystatins have been analysed.engjournal article1460-2431https://doi.org/10.1093/jxb/eru043restricted access577.112581.15CystatinCysteine proteaseLeaf senescenceNutrient recyclingProtein breakdownProteolysisBioquímica (Biología)Biología molecular (Biología)Fisiología vegetal (Biología)2403 Bioquímica2409.92 Genética Molecular de Plantas