Galindo Izquierdo, MaríaSantiago, BegoñaAlcami, JoseRivero, M.Martín Serrano, J.Pablos Álvarez, José Luis2025-01-142025-01-142001-01Galindo M, Santiago B, Alcami J, Rivero M, Martín-Serrano J, Pablos JL. Hypoxia induces expression of the chemokines monocyte chemoattractant protein-1 (MCP-1) and IL-8 in human dermal fibroblasts. Clin Exp Immunol. 2001 Jan;123(1):36-41.0009-910410.1046/j.1365-2249.2001.01412.xhttps://hdl.handle.net/20.500.14352/114212Hypoxia is an important factor in the pathophysiology of vascular and inflammatory diseases. Leucocyte infiltration, as a consequence of adhesion molecule up-regulation and chemokine release, is a prominent feature of these diseases. The objective of our study was to investigate the potential role of resident fibroblasts in hypoxia-induced chemotactic responses. We show that MCP-1 and IL-8 mRNA are specifically induced by hypoxia in dermal fibroblasts. This response is paralleled by increased NF-kappaB p65/p50 binding activity, and it is inhibited by pretreatment with N-acetyl-L-cysteine. MCP-1 secreted by fibroblasts is chemotactic for monocytic cells and this activity is significantly increased by hypoxia. Chemotactic index correlates with MCP-1 protein levels and is significantly decreased by neutralizing anti-MCP-1 MoAb. These findings demonstrate the ability of resident fibroblasts to mediate chemotaxis of leucocytes through the release of chemokines in response to hypoxia. Our data point to MCP-1 as an important component in this response, and therefore it may be a potential target in inflammatory responses associated with hypoxia.engjournal article1365-224910.1046/j.1365-2249.2001.01412.x11167995https://academic.oup.com/cei/article/123/1/36/6478200?login=truehttps://pubmed.ncbi.nlm.nih.gov/11167995/restricted access612.017FibroblastsFree radicalsChemokinesInflammationChemotaxisCiencias BiomédicasInmunología32 Ciencias Médicas2412 Inmunología