Figuero Ruiz, ElenaSánchez Beltrán, María Del CarmenCuesta Frechoso, SusanaTejerina, Jose MaríaDel Castro, Jose AntonioGutiérrez, Jose MaríaHerrera González, DavidSanz Alonso, Mariano2024-01-232024-01-232011Figuero E, Sánchez-Beltrán M, Cuesta-Frechoso S, Tejerina JM, del Castro JA, Gutiérrez JM, Herrera D, Sanz M. Detection of periodontal bacteria in atheromatous plaque by nested polymerase chain reaction. J Periodontol. 2011 Oct;82(10):1469-770022-349210.1902/jop.2011.100719https://hdl.handle.net/20.500.14352/94716Background: In recent years, increasing evidence regarding the potential association between periodontal diseases and cardiovascular diseases has been identified. The available evidence underlines the importance of detecting periodontal pathogens on atheromatous plaque as the first step in demonstrating the causal relationship between the two conditions. The main aim of this investigation is to detect periodontitis-associated bacteria from carotid artery atheromatous plaque from patients who received an endarterectomy using strict sample procurement and laboratory procedures. Methods: Atheromatous plaque from endarterectomies from carotid arteries were scraped and homogenized, and bacterial DNA was extracted. To obtain a representative concentration of amplicons, two amplifications of the bacterial 16S ribosomal-RNA gene were carried out for each sample with universal eubacteria primers by a polymerase chain reaction (PCR). A nested PCR with specific primers for the target bacteria was performed next. Statistical tests included the χ(2) test. Results: Forty-two atheromatous plaque were analyzed. All of them were positive for ≥1 target bacterial species. The bacterial species most commonly found was Porphyromonas gingivalis (78.57%; 33 of 42), followed by Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) (66.67%; 28 of 42), Tannerella forsythia (previously T. forsythensis) (61.90%; 26 of 42), Eikenella corrodens (54.76%; 23 of 42), Fusobacterium nucleatum (50.00%; 21 of 42), and Campylobacter rectus (9.52%; four of 42). The simultaneous presence of various bacterial species within the same specimen was a common observation. Conclusion: Within the limitations of this study, the presence of DNA from periodontitis-associated bacteria in carotid artery atheromatous plaque retrieved by endarterectomy is confirmed.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article1943-3670https://doi.org/10.1902/jop.2011.10071921453047restricted access616.314616.314.17-008.1611.1AtherosclerosisCardiovascular diseasesMicrobiologyPeriodontal diseasesPolymerase chain reactionPorphyromonas gingivalisOdontología (Odontología)Sistema cardiovascularPeriodoncia32 Ciencias Médicas3213.13 Ortodoncia-Estomatología3207.04 Patología Cardiovascular