González Fernández, JuanRodero Martínez, MartaDaschner, ÁlvaroCuéllar Del Hoyo, María Del Carmen2024-01-112024-01-112014González-Fernández, J., Rodero, M., Daschner, A. et al. New insights into the allergenicity of tropomyosin: a bioinformatics approach. Mol Biol Rep 41, 6509–6517 (2014). https://doi.org/10.1007/s11033-014-3534-60301-485110.1007/s11033-014-3534-6https://hdl.handle.net/20.500.14352/92457The invertebrate panallergen tropomyosin is a protein with an extremely simple folding. This makes it a perfect target for investigating structural differences between invertebrate and vertebrate tropomyosins, which are not considered allergenic. Phylogenetic and sequence analyses were conducted in order to explore the differences in primary structure between several tropomyosins and to promote an experimental development in the field of food allergy, based on the study of tropomyosin. The phylogenetic analyses showed that tropomyosin is a useful evolutionary marker. The phylogenetic trees obtained with tropomyosin were not always phylogenetically correct, but they might be useful for allergen avoidance by tropomyosin allergic individuals. Sequence analyses revealed that the probability of alpha helix folding in invertebrate tropomyosins was lower than in all the studied vertebrate ones, except for the Atlantic bluefin tuna Thunnus thynnus tropomyosin. This suggested that the lack of alpha helix folding may be involved in the immunogenicity of tropomyosins. More specifically, the regions adjacent to the positions 133-135 and 201 of the invertebrate tropomyosins, presented lower probability of alpha helix folding than those of vertebrates and are candidates to be responsible for their allergenicity.engjournal article1573-4978https://doi.org/10.1208/s12249-019-1552-324985979restricted access611.081.1Anisakis simplexAllergyBioinformaticsEpitopesEvolutionary MarkerTropomyosinBioquímica (Farmacia)Parasitología (Farmacia)Bioinformática3207.12 Parasitología