Aranaz Martín, AliciaRomero Martínez, BeatrizMontero Serra, NataliaÁlvarez Sánchez, JulioBezos Garrido, JavierJuan Ferré, Lucía DeMateos García, Ana IsabelDomínguez Rodríguez, Lucas José2024-01-172024-01-172004-06-23Aranaz, A., Romero, B., Montero, N., Alvarez, J., Bezos, J., de Juan, L., Mateos, A., & Domínguez, L. (2004). Spoligotyping profile change caused by deletion of a direct variable repeat in a Mycobacterium tuberculosis isogenic laboratory strain. Journal of clinical microbiology, 42(11), 5388–5391. https://doi.org/10.1128/JCM.42.11.5388-5391.20040095-11371098-660X10.1128/JCM.42.11.5388-5391.2004https://hdl.handle.net/20.500.14352/93502Autor de correspondencia: Alicia Aranaz; 2004, American Society for Microbiology. All Rights Reserved. Financiación: Ramon y Cajal Program (Spanish Ministry of Science and Technology).Pproject AGL 2001-2029 of the Spanish Ministry of Science and Technology and by the Spanish Ministry of Agriculture, Fisheries and Food (MAPA).Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.engAttribution-NonCommercial-NoDerivatives 4.0 Internationaljournal articlehttps://journals.asm.org/doi/10.1128/jcm.42.11.5388-5391.2004open access579.62Microbiología (Veterinaria)3109.05 Microbiología