Caso Fernández, Javier RubénHurtado Moreno, OliviaPereira, Marta P.García Bueno, BorjaMenchén Viso, Luis AlbertoAlou Cervera, LuisGómez-Lus Centelles, María LuisaMoro Sánchez, María ÁngelesLizasoaín Hernández, IgnacioLeza Cerro, Juan Carlos2024-07-242024-07-242009-04Caso JR, Hurtado O, Pereira MP, García-Bueno B, Menchén L, Alou L, Gómez-Lus ML, Moro MA, Lizasoain I, Leza JC. Colonic bacterial translocation as a possible factor in stress-worsening experimental stroke outcome. Am J Physiol Regul Integr Comp Physiol. 2009 Apr;296(4):R979-85.0363-611910.1152/ajpregu.90825.2008https://hdl.handle.net/20.500.14352/107128Stress is known to be one of the risk factors of stroke, but only a few experimental studies have examined the possible mechanisms by which prior stress may affect stroke outcome. In stroke patients, infections impede neurological recovery and increase morbidity as well as mortality. We previously reported that stress induces a bacterial translocation and that prior immobilization stress worsens experimental stroke outcome through mechanisms that involve inflammatory mediators such as release of proinflammatory cytokines and enzyme activation. We now investigate whether bacterial translocation from the intestinal flora of rats with stress before experimental ischemia is involved in stroke outcome. We used an experimental paradigm consisting of exposure of Fischer rats to repeated immobilization sessions before permanent middle cerebral artery occlusion (MCAO). The presence of bacteria and the levels and expression of different mediators involved in the bacterial translocation were analyzed. Our results indicate that stress before stroke is related to the presence of bacteria in different organs (mesenteric nodes, spleen, liver, and lung) after MCAO and increases inflammatory colonic parameters (such as cyclooxygenase-2, inducible nitric oxide synthase, and myeloperoxidase), but decreases colonic immunoglobulin A, and these results are correlated with colonic inflammation and bacterial translocation. Understanding the implication of bacterial translocation during stress-induced stroke worsening is of great potential clinical relevance, given the high incidence of infections after severe stroke and their main role in mortality and morbidity in stroke patients.engjournal article1522-1490https://doi.org/10.1152/ajpregu.90825.2008https://journals.physiology.org/doi/full/10.1152/ajpregu.90825.2008restricted access611.02615stressstrokebacterial translocationintestinal barrierstroke outcomeFarmacología (Medicina)Microbiología médica2414 Microbiología3209 Farmacología