Villegas Díaz, María RocíoBaeza, AlejandroVallet Regí, María Dulce Nombre2023-06-182023-06-182015-11-041944-824410.1021/acsami.5b07116https://hdl.handle.net/20.500.14352/23171ORCID 0000-0002-9042-8865 (Alejandro Baeza) RESEARCHER ID K-8193-2014 (Alejandro Baeza) RESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí)Poor penetration of drug delivery nanocarriers within dense extracellular matrices constitutes one of the main liabilities of current nanomedicines. The conjugation of proteolytic enzymes on the nanoparticle surface constitutes an attractive alternative. However, the scarce resistance of these enzymes against the action of proteases or other aggressive agents present in the bloodstream strongly limits their application. Herein, a novel nanodevice able to transport proteolytic enzymes coated with an engineered pH-responsive polymeric is presented. This degradable coat protects the housed enzymes against proteolytic attack at the same time that it triggers their release under mild acidic conditions, usually present in many tumoral tissues. These enzyme nanocapsules have been attached on the surface of mesoporous silica nanoparticles, as nanocarrier model, showing a significatively higher penetration of the nanopartides within 3D collagen matrices which housed human osteosarcoma cells (HOS). This strategy can improve the therapeutic efficacy of the current nanomedicines, allowing a more homogeneous and deeper distribution of the therapeutic nanosystems in cancerous tissues.spajournal articleopen access546615.46Tumoral tissue penetrationCollagenase nanocapsulesNanomedicineMesoporous silica nanodevices and hybrid nanocarriersMaterialesQuímica inorgánica (Química)3312 Tecnología de Materiales2303 Química Inorgánica