Vilas, José LuisTagare, Hemant D.Vargas Balbuena, JavierCarazo, Jose MaríaSorzano, Carlos Óscar S.2026-01-082026-01-082020-01-02Vilas, J.L., Tagare, H.D., Vargas, J. et al. Measuring local-directional resolution and local anisotropy in cryo-EM maps. Nat Commun 11, 55 (2020). https://doi.org/10.1038/s41467-019-13742-w10.1038/s41467-019-13742-whttps://hdl.handle.net/20.500.14352/129693© The Author(s) 2020. Supplementary information is available for this paper at https://doi.org/10.1038/s41467019-13742-w. R01GM114051. RGPIN-2018-04813 NC-253837The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article2041-1723https://doi.org/10.1038/s41467-019-13742-whttps://www.nature.com/articles/s41467-019-13742-wopen access535004.91Nuclear instrumentsÓptica (Física)2209.90 Tratamiento Digital. Imágenes