Fuente, Hortensia de laMittelbrunn, MaríaSánchez-Martín, LorenaVicente-Manzanares, MiguelLamana Domínguez, AmaliaPardi, RuggeroCabañas Gutiérrez, CarlosSánchez-Madrid, Francisco2024-01-192024-01-192005De La Fuente, Hortensia, et al. «Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–Mediated Initial T-Cell Scanning». Molecular Biology of the Cell, vol. 16, n.o 7, julio de 2005, pp. 3314-22. https://doi.org/10.1091/mbc.e05-01-0005.1939-458610.1091/mbc.e05-01-0005https://hdl.handle.net/20.500.14352/93998Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells.engjournal articlehttps://doi.org/10.1091/mbc.e05-01-0005open access576.3577.27T-cellSynaptic ClustersMHC Class II MoleculesBiología celular (Biología)Inmunología2412 Inmunología2407 Biología Celular