Ruiz Valdepeñas Montiel, VíctorCampuzano Ruiz, SusanaConzuelo, FelipeTorrente Rodríguez, Rebeca MagnoliaGamella Carballo, MaríaReviejo García, Ángel JulioPingarrón Carrazón, José Manuel2024-01-252024-01-252015Ruiz-Valdepeñas Montiel V, Campuzano S, Conzuelo F, Torrente-Rodríguez RM, Gamella M, Reviejo AJ, Pingarrón JM. Electrochemical magnetoimmunosensing platform for determination of the milk allergen β-lactoglobulin. Talanta. 2015 Jan;131:156-62. doi: 10.1016/j.talanta.2014.07.076. Epub 2014 Aug 5. PMID: 25281087.0039-914010.1016/j.talanta.2014.07.076https://hdl.handle.net/20.500.14352/95450A very sensitive magnetoimmunosensor for the determination of β-lactoglobulin (β-LG) is reported in this work. A sandwich configuration involving covalent immobilization of the capture antibody (antiβ-LG) onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a horseradish peroxidase labeled antibody (HRP-antiβ-LG), is used. The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as electron transfer mediator and hydrogen peroxide as the enzyme substrate. The β-LG magnetoimmunosensor exhibited a wide range of linearity (2.8–100 ng/mL) and a low detection limit of 0.8 ng/mL (20 pg in 25 μL sample). The magnetoimmunosensing platform was successfully applied for the detection of β-LG in different types of milk without any matrix effect after just a sample dilution. The results correlated properly with those provided by a commercial ELISA method offering a truthful analytical screening tool. These features make the developed methodology a promising alternative in the development of user-friendly devices for on-site determination of β-LG in dairy products.engjournal articlehttps://doi.org/10.1016/j.talanta.2014.07.076https://pubmed.ncbi.nlm.nih.gov/25281087/restricted access543β-LactoglobulinAmperometric magnetoimmunosensorsMagnetic beadsMilkQuímica2301 Química Analítica