Serafín González-Carrato, VerónicaValverde De La Fuente, AlejandroMartínez-García, GonzaloMartínez-Periñán, EmilianoComba, FaustoGarranzo-Asensio, MaríaBarderas Manchado, RodrigoYáñez-Sedeño Orive, PalomaCampuzano Ruiz, SusanaPingarrón Carrazón, José Manuel2024-01-252024-01-252019Serafín V, Valverde A, Martínez-García G, Martínez-Periñán E, Comba F, Garranzo-Asensio M, Barderas R, Yáñez-Sedeño P, Campuzano S, Pingarrón J.M. Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potential. Sens. Actuators, B. 2019 Enero 3; 284: 711-220925-400510.1016/j.snb.2019.01.012https://hdl.handle.net/20.500.14352/95375In this work, the development of the first integrated electrochemical immunosensor for the determination of the IL-13Rα2 is reported. The strategy involves the immobilization of a biotinylated capture antibody onto streptavidin-modified screen-printed electrodes through grafting with p-aminobezoic acid (p-ABA) and further activation using EDC/Sulfo-NHS chemistry. A hybrid nanomaterial composed of multiwalled carbon nanotubes (MWCNTs) and Graphene Quantum Dots (GQDs) was used as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the system H2O2/hydroquinone (HQ) achieved a linear calibration plot ranging from 2.7 to 100 ng mL−1 IL-13sRα2, with a LOD value of 0.8 ng mL−1. The immunosensor showed an excellent selectivity and was successfully applied to the determination of the target receptor directly in small amounts of raw cellular lysates and extracts of paraffin-embedded tissues from patients diagnosed with colorectal cancer at different stages.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potentialjournal articlehttps://doi.org/10.1016/j.snb.2019.01.012https://produccioncientifica.ucm.es/grupos/4975/colaboracion/organizaciones/1230open access544.6Graphene quantum dotsIL-13Rα2CancerElectrochemical immunosensorParaffin-embedded tissuesQuímica analítica (Química)2301 Química Analítica