Fresco-Taboada, AlbaSerra, ImmacolataArroyo Sánchez, MiguelFernández-Lucas, JesúsDe La Mata Riesco, Mª IsabelTerreni, Marco2023-06-182023-06-182016-010920-586110.1016/j.cattod.2015.06.032https://hdl.handle.net/20.500.14352/23393The immobilization of Bacillus psychrosaccharolyticus nucleoside 2 -deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis oftrifluridine and decytabine obtaining conversions ranging from 50% to 76%.engDevelopment of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabinejournal articlehttp://www.sciencedirect.com/science/article/pii/S0920586115004836restricted access577.15Nucleoside 2′-deoxyribosyltransferaseBiotransformationsEnzyme immobilizationNucleoside analoguesDecytabineTrifluridineBioquímica (Biología)2302 Bioquímica