Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus

Castellanos, ElenaAranaz Martín, AliciaDe Buck, Jeroen2023-06-202023-06-202010-041098-660X10.1128/JCM.02484-09https://hdl.handle.net/20.500.14352/45190High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.engRapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locusjournal articlehttp://jcm.asm.org/content/48/4/1474.fullrestricted accessVeterinaria3109 Ciencias Veterinarias