Cañadas Benito, OlgaCasals Carro, María CristinaKleinchmidt, Jorg2024-02-092024-02-092012Cañadas O, Casals C. Differential scanning calorimetry of protein-lipid interactions. In Lipid-protein interactions: Methods and Protocols. Methods Mol Biol. 974:55-71. J. Kleinchmidt (ed.). 2013, doi: 10.1007/978-1-62703-275-9_4, Print ISBN: 978-1-62703-274-2. Editorial: Springer Science+Business Media, New York978-1-62703-274-210.1007/978-1-62703-275-9_4https://hdl.handle.net/20.500.14352/100928This work was supported by the Ministerio de Ciencia e Innovacion (SAF2009-07810) and Instituto de Salud Carlos III (cibeRESCB06/06/0002).Differential scanning calorimetry (DSC) is a highly sensitive non-perturbing technique for measuring the thermodynamic properties of thermally induced transitions. This technique is particularly useful for the characterization of lipid/protein interactions. This chapter presents an introduction to DSC instrumentation, basic theory, and methods and describes DSC applications for characterizing protein effects on model lipid membranes. Examples of the use of DSC for the evaluation of protein effects on modulation of membrane domains and membrane stability are given.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalbook parthttps://doi.org/10.1007/978-1-62703-275-9_4metadata only access577.1Differential scanning calorimetryLipidsProteinsLipid–protein interactionAntimicrobial peptidesData analysisBioquímica (Química)2302 Bioquímica