Marquina Díaz, DomingoBarroso, J.Santos de la Sen, AntonioMartínez Peinado, José2024-09-182024-09-182001Marquina, D., et al. «Production and Characteristics of Debaryomyces Hansenii Killer Toxin». Microbiological Research, vol. 156, n.o 4, 2001, pp. 387-91. DOI.org (Crossref), https://doi.org/10.1078/0944-5013-00117.0944-501310.1078/0944-5013-00117https://hdl.handle.net/20.500.14352/108224The optimal conditions for the production of the killer toxin of Debaryomyces hansenii CYC 1021 have been studied. The lethal activity of the killer toxin increased with the presence of NaCl in the medium used for testing the killing action. Production of the killer toxin was stimulated in the presence of proteins of complex culture media. Addition of nonionic detergents and other additives, such as dimethylsulfoxide enhanced killer toxin production significantly. Killer toxin secretion pattern followed the growth curve and reached its maximum activity at the early stationary phase. Optimal stability was observed at pH 4.5 and temperatures up to 20 °C. Above pH 4.5 a steep decrease of the stability was noted. The activity was hardly detectable at pH 5.1.engjournal article1618-0623https://doi.org/10.1078/0944-5013-0011711770858https://www.sciencedirect.com/science/article/pii/S094450130470052X?via%3Dihubrestricted access579.6663.12Debaryomyces hanseniiKiller toxinProductionHalotoleranceMicrobiología (Biología)Bioquímica (Biología)2414 Microbiología2403 Bioquímica