Palacios López, María MerceMartín Gálvez, DavidPulido Delgado, FranciscoDolores Barón, MaríaArriero Higueras, Elena2025-05-162025-05-162025Palacios, M., Martín-Gálvez, D., Pulido, F., Dolores Barón, M. and Arriero, E. (2025), Adaptive immunity modulation linked to migratory behaviour in two partially migratory hoopoe Upupa epops populations. J Avian Biol, 2025: e03383. https://doi.org/10.1111/jav.033830908-885710.1111/jav.03383https://hdl.handle.net/20.500.14352/120142This study was funded by research grant no. PID2020-116934GB-I00 from the Spanish Ministry of Science and Innovation. MP was funded by a predoctoral contract PRE2021-099265 associated to the research grant no. PID2020-116934GB-I00, and MDB was funded by a predoctoral contract PRE2021-099473, associated to the research grant no. PID2020-117429GB-C21.Ecophysiology has enhanced our understanding of avian migration, yet many aspects of how these processes interrelate are still unclear. Partially migratory populations provide an ideal framework for its study in the wild, since resident and migratory individuals coexist temporarily in the same area and face similar selection pressures. We focused on two Iberian populations of Eurasian hoopoe Upupa epops, a trans-Saharan long-distance migrant, to explore the links between the immune system and migratory behaviour. We determined the migratory status of individual hoopoes using stable isotope analysis of deuterium (2H) and measured a number of immunological parameters, including estimates of innate and adaptive immunity, as well as body condition, and muscle and fat stores. Our results indicate that resident hoopoes had higher IgY levels and higher muscle and fat stores compared to migrants during the breeding season. Moreover, we found seasonal variation in leukocyte profiles of resident birds, with higher heterophil/lymphocyte (H/L) ratios in winter than during the breeding season. We observed significantly higher H/L ratios and complement activity in resident males than in resident females, but not within migratory birds. Overall, we show differences in immune response linked to migratory behaviour in partial migratory populations. This study contributes to unraveling the associations between physiological status and migratory behaviour and ultimately helps to understand how different migratory strategies are maintained in partially migratory populations.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article1600-048Xhttps://doi.org/10.1111/jav.03383https://nsojournals.onlinelibrary.wiley.com/doi/10.1111/jav.03383open access598.7591.5591.15BirdsEcoimmunologyImmunoglobulinsPartial migrationSeasonal variationStable isotopesZoologíaComportamiento animalEcología (Biología)AvesInmunología2401 Biología Animal (Zoología)2401.20 Ornitología2401.02 Comportamiento Animal3109.03 Inmunología2401.06 Ecología Animal