Santos-Lozano, AlejandroValenzuela, Pedro L.Llavero, FranciscoLista, SimoneCarrera-Bastos, PedroHampel, HaraldPareja-Galeano, HeliosGonzález Gálvez, BeatrizLópez, Juan AntonioVázquez, JesúsEmanuele, EnzoZugaza, José L.Lucia, Alejandro2024-06-112024-06-112020-02-251945-458910.18632/aging.102826https://hdl.handle.net/20.500.14352/104842Healthy aging depends on a complex gene-environment network that is ultimately reflected in the expression of different proteins. We aimed to perform a comparative analysis of the plasma proteome of healthy centenarians (n=9, 5 women, age range 100-103 years) with a notably preserved ambulatory capacity (as a paradigm of 'successful' aging), and control individuals who died from a major age-related disease before the expected life expectancy (n=9, 5 women, age range: 67-81 years), and while having impaired ambulatory capacity (as a paradigm of 'unsuccessful' aging). We found that the expression of 49 proteins and 86 pathways differed between the two groups. Overall, healthy centenarians presented with distinct expression of proteins/pathways that reflect a healthy immune function, including a lower pro-inflammatory status (less 'inflammaging' and autoimmunity) and a preserved humoral immune response (increased B cell-mediated immune response). Compared with controls, healthy centenarians also presented with a higher expression of proteins involved in angiogenesis and related to enhanced intercellular junctions, as well as a lower expression of proteins involved in cardiovascular abnormalities. The identification of these proteins/pathways might provide new insights into the biological mechanisms underlying the paradigm of healthy aging.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.18632/aging.102826open access577.1577.2proteomicsimmune systemsenescenceelderlyhealthy agingBiología molecular (Farmacia)Bioquímica (Farmacia)32 Ciencias Médicas