Muñoz Colmenero, Ana MartaBaroja Careaga, IgorKovačić, MarinFilipi, JanjaPuškadija, Z.Kezić, N.Estonba, AndoneBüchler, RalphZarraonaindia, Iratxe2024-10-242024-10-242020-07Muñoz-Colmenero M, Baroja-Careaga I, Kovačić M, Filipi J, Puškadija Z, Kezić N, Estonba A, Büchler R, Zarraonaindia I. Differences in honey bee bacterial diversity and composition in agricultural and pristine environments – a field study. Apidologie 2020;51:1018–37. https://doi.org/10.1007/s13592-020-00779-w.0044-843510.1007/s13592-020-00779-whttps://hdl.handle.net/20.500.14352/109475This research was supported by the Genomic Resources research group (http://www.genomic-resources.eus) of the Basque University System (IT1014-16) supported by the Department of Education, Universities and Research of the Basque government.Agrochemicals and biocides are suspected to cause a dysbiosis of honey bee microbiota, decreasing colonies ability to respond to the environment. As a first step to investigate agriculture and beekeeping impact, hives bacteriomes from an anthropized environment (Agri-env) were compared to that of pristine’s (Prist-env). 16S rRNA sequencing evidenced differences in richness and composition between sample types (Gut (G), Brood (B), Bee-bread (BB)) and environments. Higher opportunist loads and shifts toward taxa capable of metabolizing insecticides were observed in G and B at Agri-env, while beneficial bacteria were enriched in Prist-env. Bacteria in BB did not differ, the acidity of the niche outweighing the influence of external factors. Results showed the environment plays a major role in shaping honey bee microbiota, the agricultural realm inducing a bacterial disruption that would let to colonies vulnerability. In contrast, a less susceptible bee will be promoted in less anthropized locations.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article1297-9678https://doi.org/10.1007/s13592-020-00779-whttps://link.springer.com/article/10.1007/s13592-020-00779-w#Abs1open access579.62595.799638.1Honey beeBacteriomeEnvironmentAgricultureBeekeepingMicrobiología (Biología)Insectos2413 Biología de Insectos (Entomología)2414 Microbiología3104 Producción Animal