Daavari, MortezaAtapour, MasoudMohedano Sánchez, MartaMatykina, EndzheArrabal Durán, Raúl2024-07-052024-07-052023-09-16Daavari, M.; Atapour, M.; Mohedano, M.; Matykina, E.; Arrabal, R.; Nesic, D. Biological Performance of Duplex PEO + CNT/PCL Coating on AZ31B Mg Alloy for Orthopedic and Dental Applications. J. Funct. Biomater. 2023, 14, 475. https://doi.org/10.3390/jfb140904752079-498310.3390/jfb14090475https://hdl.handle.net/20.500.14352/105718To regulate the degradation rate and improve the surface biocompatibility of the AZ31B magnesium alloy, three different coating systems were produced via plasma electrolytic oxidation (PEO): simple PEO, PEO incorporating multi-walled carbon nanotubes (PEO + CNT), and a duplex coating that included a polycaprolactone top layer (PEO + CNT/PCL). Surfaces were characterized by chemical content, roughness, topography, and wettability. Biological properties analysis included cell metabolism and adhesion. PEO ± CNT resulted in an augmented surface roughness compared with the base material (BM), while PCL deposition produced the smoothest surface. All surfaces had a contact angle below 90°. The exposure of gFib-TERT and bmMSC to culture media collected after 3 or 24 h did not affect their metabolism. A decrease in metabolic activity of 9% and 14% for bmMSC and of 14% and 29% for gFib-TERT was observed after 3 and 7 days, respectively. All cells died after 7 days of exposure to BM and after 15 days of exposure to coated surfaces. Saos-2 and gFib-TERT adhered poorly to BM, in contrast to bmMSC. All cells on PEO anchored into the pores with filopodia, exhibited tiny adhesion protrusions on PEO + CNT, and presented a web-like spreading with lamellipodia on PEO + CNT/PCL. The smooth and homogenous surface of the duplex PEO + CNT/PCL coating decreased magnesium corrosion and led to better biological functionality.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articleopen access66biodegradable Mg-based implantplasma electrolyte oxidation (PEO)multi-walled carbon nanotubes (MWCNT)polycaprolactone (PCL)cell adhesioncell metabolismMateriales3303 Ingeniería y Tecnología Químicas