Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A

Ruge, Christian ArnoldKirch, JulianCañadas Benito, OlgaSchneider, MarcPérez Gil, JesúsSchaefer, Ulrich FriedrichCasals Carro, María CristinaLehr, Claus Michael2024-01-222024-01-222011Ruge CA, Kirch K, Cañadas O, Schneider M, Pérez-Gil J, Schaefer UF, Casals C, Lehr CM. Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A. Nanomedicine: Nanotechnology, Biology and Medicine 2011 Dec;7:690-6931549-963410.1016/j.nano.2011.07.009https://hdl.handle.net/20.500.14352/94434From the clinical editor: In this nano-toxicology study of inhaled nanoparticles, the authors investigated the effect of pulmonary surfactant protein A on the interaction between nanoparticles and alveolar macrophages utilizing magnetite nanoparticles coated with different polymers (starch, carboxymethyldextran, chitosan, poly-maleic-oleic acid, phosphatidylcholine). Cellular binding and uptake of nanoparticles increased for nanoparticles treated with SP-A, whereas albumin, the prevailing protein in plasma, led to a significant decrease.Understanding the bio-nano interactions in the lungs upon the inhalation of nanoparticles is a major challenge in both pulmonary nanomedicine and nanotoxicology. To investigate the effect of pulmonary surfactant protein A (SP-A) on the interaction between nanoparticles and alveolar macrophages, we used magnetite nanoparticles (110-180 nm in diameter) coated with different polymers (starch, carboxymethyldextran, chitosan, poly-maleic-oleic acid, phosphatidylcholine). Cellular binding and uptake of nanoparticles by alveolar macrophages was increased for nanoparticles treated with SP-A, whereas albumin, the prevailing protein in plasma, led to a significant decrease. A significantly different adsorption pattern of SP-A, compared to albumin was found for these five different nanomaterials. This study provides evidence that after inhalation of nanoparticles, a different protein coating and thus different biological behavior may result compared to direct administration to the bloodstreamengUptake of nanoparticles by alveolar macrophages is triggered by surfactant protein Ajournal articlehttps://doi.org/10.1016/j.nano.2011.07.009restricted access577.112616.2615.4SP-AProtein adsorptionMacrophage clearanceBioquímica (Biología)Biología molecular (Química)Farmacología (Medicina)2302.21 Biología Molecular2403 Bioquímica2302.22 Farmacología Molecular