Barderas Manchado, RodrigoCampuzano Ruiz, SusanaGamella Carballo, MaríaMuñoz San Martín, CristinaPedrero Muñoz, MaríaPingarrón Carrazón, José ManuelSerafín González-Carrato, VerónicaValverde De La Fuente, AlejandroYáñez-Sedeño Orive, Palomaben Hassine, AmiraGarranzo Asensio, MaríaRaouafi, Noureddine2026-02-122026-02-122019-10-01Valverde, Alejandro, et al. «Dual Amperometric Immunosensor for Improving Cancer Metastasis Detection by the Simultaneous Determination of Extracellular and Soluble Circulating Fraction of Emerging Metastatic Biomarkers». Electroanalysis, vol. 32, n.o 4, abril de 2020, pp. 706-14. DOI.org (Crossref), https://doi.org/10.1002/elan.201900506.10.1002/elan.201900506https://hdl.handle.net/20.500.14352/132187This paper reports the development of a dual immunosensor using magnetic microcarriers (MBs) and amperometric transduction at dual screen-printed carbon electrodes (SPdCEs) for the simultaneous determination of two biomarkers: interleukin-13 receptor α2 (IL-13Rα2) and E-cadherin (E-CDH), with both extracellular and soluble fraction; oncogenic and tumor suppressor markers, respectively, of great relevance in metastatic processes. The implemented methodology involved the formation of sandwich-type immunocomplexes using specific capture antibodies immobilized onto carboxylic acid magnetic microbeads (HOOC-MBs), and biotiny-lated detector antibodies labeled with strepta-vidin horseradish peroxidase conjugates (Strep-HRP). The amperometric detection was performed by addition of hydrogen peroxide in the presence of hydroquinone (HQ) as the redox mediator. The dual immunosensing platform provided linear calibration ranges suitable for the determination of both biomarkers in liquid and solid clinical specimens as well as excellent selectivity against other cancer biomarkers. This simple handling dual bioplatform was applied to the determination of the soluble and extracellular fraction of the target biomarkers in serum and paraffined-embedded tissues from colorectal cancer (CRC) patients diagnosed at different tumor grade. The obtained results reveal great potential of this configuration to improve the reliability in diagnosing metastatic CRC.engjournal articlehttps://doi.org/10.1002/elan.201900506https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elan.201900506restricted access543Dual electrochemical immunosensorMetastasis-related biomarkersIL-13Rα2E-CDHExtracellular and soluble fractionQuímica2301 Química Analítica