Carrión Herrero, Francisco JavierDomínguez Bernal, Gustavo RamónMartínez Rodrigo, AbelMas Zubiri, AliciaBlanco Gutiérrez, María Del MarOrden Gutiérrez, José AntonioFuente López, Ricardo De La2024-01-182024-01-182017-10Domínguez-Bernal, G., Martínez-Rodrigo, A., Mas, A., Blanco, M. M., Orden, J. A., De La Fuente, R., & Carrión, J. (2017). Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells. Comparative immunology, microbiology and infectious diseases, 54, 13–19. https://doi.org/10.1016/j.cimid.2017.07.00210.1016/j.cimid.2017.07.002https://hdl.handle.net/20.500.14352/93736Here, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection.engAttribution 4.0 Internationaljournal article0147-9571https://www.sciencedirect.com/science/article/pii/S0147957117300577open access636.09Dendritic cellsHisAK70LeishmaniosisSalmonellaVaccinationVeterinaria24 Ciencias de la Vida