Bruna García, EvaIsabel Redondo, BeatrizSabater Muñoz, BeatrizMiguel Castro, Marta2024-05-242024-05-242023-08-28Eva Bruna-García, Beatriz Isabel-Redondo, Beatriz Sabater-Munoz, Marta Miguel-Castro, Assessment of antioxidant bioactive properties of iberian dry-cured ham fat-derived essences using the yeast Saccharomyces cerevisiae as experimental model, LWT, Volume 186, 2023, 115235, https://doi.org/10.1016/j.lwt.2023.115235.0023-643810.1016/j.lwt.2023.115235https://hdl.handle.net/20.500.14352/1044242023 Acuerdos transformativos CRUEA new essence derived from Iberian dry-cured ham trimmed fat, with high content of mono- and poly-unsaturated fatty acids, and with antioxidant properties may act as protective agent counteracting the cellular effects of an oxidative insult. We used Saccharomyces cerevisiae, as in vivo experimental model to determine antioxidant properties of essences T30, T60 and T80. Antioxidant capacity was measured as recovery of yeast growth parameters and survivorship, as proxy of systemic cellular response to oxidative stress, when compared to unstressed or stressed cells treated with known antioxidants (vitamin C and resveratrol). At lower dose (1 μl), treated yeasts recovered growth rate (rT30 = 0.252 ± 0.016, rT60 = 0.224 ± 0.054, rT80 = 0.244 ± 0.032 h−1) and increased the number of relative survivors (RST30 = 1.494 ± 0.686, RST60 = 1.384 ± 0.405, RST80 = 1.720 ± 0.650) when compared to oxidative stressed yeasts (r = 0.093 ± 0.029 h−1, RS = 1.000 ± 0.000). The developed essences (T30, T60 and T80) counteract oxidative damage, with no-statistical significantly differences between essences, in a dose-dependent manner. In conclusion, the developed essences could be used as a functional food ingredient to relief oxidative stress, either raising from the onset of various chronic diseases or from external conditions (improving skin conditions), although they need to be validated in clinical studies.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1096-1127https://doi.org/10.1016/j.lwt.2023.115235open access636.09Oxidative cellular damage and stress responseAntioxidant activityComplex lipidic matrixGrowth rate alterationsIn vivo unicellular experimental modelVeterinaria3109 Ciencias Veterinarias