Bustamante, NeliaCantarino Aragón, María HelenaArahuetes Portero, Rosa MaríaCubero Palero, Francisco JavierOrtiz, Agustín2024-01-312024-01-312005Bustamante N, Cantarino MH, Arahuetes RM, Cubero FJ, Ortiz A. Evolution of the activity of UGT1A1 throughout the development and adult life in a rat. Life Sci. 2006 Mar 6;78(15):1688-95. doi: 10.1016/j.lfs.2005.08.018. Epub 2005 Nov 28. PMID: 16310220.0024-320510.1016/j.lfs.2005.08.018https://hdl.handle.net/20.500.14352/97158Biliary excretion is the main route of disposal of bilirubin and impaired excretion results in jaundice, a well recognisable symptom of liver disease. Conjugation of bilirubin in the liver is essential for its clearance. The glucuronidation of bilirubin is catalysed by the microsomal UDP-glucuronosyltransferase UGT1A1. Patients with Crigler-Najjar syndrome type 1 and Gunn rats, mutant strain of the Wistar rats, bear an autosomal recessive disorder resulting in hyperbilirubinemia. The aim of this work is to add new data about activity of UGT1A1 during the perinatal period and adult life. The results showed that activity of UGT1A1 is detectable from day 22 of the gestation. After birth, activity of UGT1A1 gradually increases and reaches the levels of adult life. Furthermore, bilirubin azopigments have been separated and characterized by thin layer chromatography. We have found that concentration of samples by evaporation and ulterior storing at -20 degrees C seemed to be suitable for the maintenance of samples.engjournal articlehttps://doi.org/10.1016/j.lfs.2005.08.01816310220restricted accessUGT1A1 activityGlucuronidationMonoglucuronidesDiglucuronidesDiazotizationMicrosomeAzopigmentsBilirubinCiencias BiomédicasBiologíaBiología celular (Biología)Biología molecular (Biología)Gastroenterología y hepatología24 Ciencias de la Vida2401 Biología Animal (Zoología)2401.13 Fisiología Animal2407 Biología Celular2415 Biología Molecular3206.10 Enfermedades de la Nutrición