Torrente Rodríguez, Rebeca MagnoliaCampuzano Ruiz, SusanaLópez-Hernández, EvaRuiz Valdepeñas Montiel, VíctorBarderas Manchado, RodrigoGranados, RosarioSánchez-Puelles, Jose MariaPingarrón Carrazón, José Manuel2024-01-292024-01-292015Torrente-Rodríguez RM, Campuzano S, López-Hernández E, Montiel VR, Barderas R, Granados R, Sánchez-Puelles JM, Pingarrón JM. Simultaneous detection of two breast cancer-related miRNAs in tumor tissues using p19-based disposable amperometric magnetobiosensing platforms. Biosens Bioelectron. 2015 Apr 15;66:385-91. doi: 10.1016/j.bios.2014.11.047. Epub 2014 Nov 26. PMID: 25481114.0956-566310.1016/j.bios.2014.11.047https://hdl.handle.net/20.500.14352/95881Los resultados derivados de esta investigación fueron empleados por la Oficina de Transferencia de Resultados de Investigación de la UCM para la elaboración de una noticia con título: 'Un biosensor magnético detecta el cáncer de mama más agresivo'. Además, este artículo fue resaltado en numerosos sitios web, poniendo de manifiesto su indiscutible relevancia. Algunos de los sitios en los que la noticia fue publicaba se incluyen a continuación: https://www.agenciasinc.es/Noticias/Un-biosensor-magnetico-detecta-el-cancer-de-mama-mas-agresivo https://revistalvr.es/un-biosensor-magnetico-detecta-el-cancer-de-mama-mas-agresivo/ https://www.biotechmagazine.es/noticias-biotech/biosensor-magnetico-frente-al-cancer-de-mama/ https://www.infosalus.com/asistencia/noticia-biosensor-detectar-cancer-mama-mas-agresivo-made-in-spain-20150422183529.html https://www.rtve.es/noticias/20150422/biosensor-magnetico-detecta-cancer-mama-mas-agresivo-solo-15-minutos/1134380.shtml https://www.bolsamania.com/noticias/sanidad/disenan-un-biosensor-magnetico-capaz-de-detectar-precozmente-el-cancer-de-mama-mas-agresivo--699233.html http://biotech-spain.com/en/articles/un-biosensor-magn-tico-detecta-el-c-ncer-de-mama-m-s-agresivo/A novel magnetobiosensing approach for the rapid and simultaneous detection of two breast cancer-related miRs (miR-21 and miR-205) is reported. It involves the use of antimiR-21 and antimiR-205 specific probes, chitin-modified magnetic beads (Chitin-MBs), the p19 viral protein as capture bioreceptor and amperometric detection with the H2O2/hydroquinone (HQ) system at dual screen-printed carbon electrodes (SPdCEs). The use of SPdCEs allows the simultaneous independent amperometric readout for each target miR to be measured. The magnetosensor exhibited sensitive and selective detection with dynamic ranges from 2.0 to 10.0nM and detection limits of 0.6nM (6fmol) for both target miRs without any amplification step in less than 2h. The usefulness of the approach was evaluated by detecting the endogenous levels of both target miRs in total RNA (RNAt) extracted from metastatic breast cancer cell lines and human tissues.engSimultaneous detection of two breast cancer-related miRNAs in tumor tissues using p19-based disposable amperometric magnetobiosensing platformsjournal articlehttp://doi.org/10.1016/j.bios.2014.11.047restricted access543MiRNA-21MiRNA-205P19Simultaneous detectionMagnetic beadsSPdCEsCancer samplesQuímica analítica (Química)2301 Química Analítica