Bruña Fernández, RicardoPereda, Ernesto2024-02-082024-02-082021-01-082666-522010.1016/j.brain.2021.100021https://hdl.handle.net/20.500.14352/100252The estimation of functional connectivity (FC) from noninvasive electrophysiological data recorded from sensors outside the skull requires transforming these data into a source space. As the number of sensors is much lower than the number of electrophysiological sources, the brain activity is usually parcellated into anatomical regions, and the FC between each pair of regions is then estimated. In this work, we generate a set of simulated scenarios with different configurations and coupling levels between synthetic time series. Then, this simulated brain activity is converted into simulated MEG sensor-space data and reconstructed back into the source space. Last, we estimated the FC between different regions using different approaches commonly used in the literature and compared them with a novel approach. Our results show that this novel approach, based on using all the information in each region, clearly outperforms classical approaches based on a representative time series. The proposed approach is more sensitive to the level of coupling and the extent of the area synchronized, and the resulting estimate better reflects the underlying FC. Based on these results, we strongly discourage using a representative time series to summarize large brain areas' activity when calculating FC.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://www.sciencedirect.com/science/article/pii/S2666522021000010open access612.8Functional connectivityMultivariate phase synchronizationSource reconstructionNeurociencias (Medicina)2490 Neurociencias2404 Biomatemáticas