Mayol Hornero, BeatrizPrádana López, SandraGarcía, Albade la Torre, CristinaDíez, PaulaVillalonga, AnabelAnillo, CarlosVilela García, DianaSánchez Sánchez, AlfredoMartínez Ruiz, María PalomaMartínez Máñez, RamónVillalonga Santana, Reynaldo2026-01-082026-01-082024Beatriz Mayol, Sandra Pradana-López, Alba García, Cristina de la Torre, Paula Díez, Anabel Villalonga, Carlos Anillo, Diana Vilela, Alfredo Sánchez, Paloma Martínez-Ruiz, Ramón Martínez-Máñez, Reynaldo Villalonga, Self-propelled enzyme-controlled IR-mesoporous silica Janus nanomotor for smart delivery, Journal of Colloid and Interface Science, Volume 671, 2024, Pages 294-302, ISSN 0021-9797, https://doi.org/10.1016/j.jcis.2024.05.134.10.1016/j.jcis.2024.05.134https://hdl.handle.net/20.500.14352/129581Here, we report the preparation of a novel Janus nanoparticle with opposite Ir and mesoporous silica nanoparticles through a partial surface masking with toposelective modification method. This nanomaterial was employed to construct an enzyme-powered nanomachine with self-propulsion properties for on-command delivery. The cargo-loaded nanoparticle was provided with a pH-sensitive gate and unit control at the mesoporous face by first attaching boronic acid residues and further immobilization of glucose oxidase through reversible boronic acid esters with the carbohydrate residues of the glycoenzyme. Addition of glucose leads to the enzymatic production of H2O2 and gluconic acid, being the first compound catalytically decomposed at the Ir nanoparticle face producing O2 and causing the nanomachine propulsion. Gluconic acid leads to a pH reduction at the nanomachine microenvironment causing the disruption of the gating mechanism with the subsequent cargo release. This work demonstrates that enzyme-mediated self-propulsion improved release efficiency being this nanomotor successfully employed for the smart release of Doxorubicin in HeLa cancer cells.engjournal articlehttps://doi.org/10.1016/j.jcis.2024.05.134https://www.sciencedirect.com/science/article/pii/S0021979724011202restricted access543NanomotorJanusIridiumSilicaEnzymeDrugDeliveryQuímica23 Química