Del Arco, JonJordaan, JustinMoral Dardé, VerónicaFernández Lucas, Jesús2024-11-082024-11-082019Del Arco, J., Jordaan, J., Moral-Dardé, V., & Fernández-Lucas, J. (2019). Sustainable production of nucleoside analogues by a high-efficient purine 2″-deoxyribosyltransferase immobilized onto Ni2+ chelate magnetic microparticles. Bioresource Technology, 289. https://doi.org/10.1016/J.BIORTECH.2019.1217720960-852410.1016/j.biortech.2019.121772https://hdl.handle.net/20.500.14352/110370The present work aims to develop a magnetic biocatalyst for customized production of nucleoside analogues using mutant His-tagged purine 2′-deoxyribosyltransferase from Trypanosoma brucei (TbPDTV11S) immobilized onto Ni2+ chelate magnetic iron oxide porous microparticles (MTbPDTV11S). Biochemical characterization revealed MTbPDTV11S5 as optimal candidate for further studies (10,552 IU g−1; retained activity 54% at 50 °C and pH 6.5). Interestingly, MTbPDTV11S5 displayed the highest activity value described up to date for an immobilized NDT. Moreover, MTbPDTV11S5 was successfully employed in the one-pot, one-step production of different therapeutic nucleoside analogues, such as cladribine or 2′-deoxy-2-fluoroadenosine, among others. Finally, MTbPDTV11S5 proved to be stable when stored at 50 °C for 8 h and pH 6.0 and reusable up to 10 times without negligible loss of activity in the enzymatic production of the antitumor prodrug 2′-deoxy-2-fluoroadenosine.engjournal article1873-2976https://doi.org/10.1016/j.biortech.2019.121772https://www.sciencedirect.com/science/article/pii/S0960852419310028restricted access571.113.3577.1566.095Enzyme immobilizationBioprocessesNucleoside analogues2′-DeoxyribosyltransferasesBioquímica (Biología)Química industrialBiotecnología2403 Bioquímica2302.09 Enzimología2302.23 Ácidos Nucleicos3303.11 Química Industrial