Santos de la Sen, AntonioMarquina Díaz, Domingo2024-09-032024-09-032004Santos, A., y D. Marquina. «Ion Channel Activity by Pichia Membranifaciens Killer Toxin». Yeast, vol. 21, n.o 2, enero de 2004, pp. 151-62. DOI.org (Crossref), https://doi.org/10.1002/yea.1069.0749-503X10.1002/yea.1069https://hdl.handle.net/20.500.14352/107859The cytocidal effect of Pichia membranifaciens killer toxin on Candida boidinii cells was studied. The halotolerant yeast P. membranifaciens CYC 1106 produces a unique 18 kDa killer toxin that exerts its killer activity against C. boidinii IGC 3430 only in the presence of NaCl. Metabolic events associated with the loss of C. boidinii IGC 3430 viability were quantitatively identical to those known to occur with K1 killer toxin-treated sensitive strains of Saccharomyces cerevisiae. The death of sensitive cells was characterized by a leakage of potassium, an influx of sodium and a decrease in intracellular pH. These effects occurred prior to and concomitantly with cell death, indicating that they were primary effects of the action of the toxin. Here we report that this protein forms ion-permeable channels in liposome membranes. These channels are freely permeable to common physiological ions. We suggest that channel formation is the cytotoxic mechanism of action of P. membranifaciens killer toxin. The channels described here are sufficiently non-selective to mediate cell death through a discharge of cellular membrane potential and changes in ionic homeostasis. No specific effects against killer toxin-treated sensitive cells were observed when the cell cycle was analysed.engjournal article1097-0061https://doi.org/10.1002/yea.106914755640https://onlinelibrary.wiley.com/doi/10.1002/yea.1069restricted access579.6663.12:576Pichia membranifaciensKiller toxinPatch-clampIon channel activityMicrobiología (Biología)Biología celular (Biología)2414 Microbiología2407 Biología Celular