Franco Mena, DanielaDe la Riva, IgnacioVega Yánez, Mateo A.Székely, PaulAmador, LuisBatallas, DiegoReyes Puig, Juan P.Cisneros Heredia, Diego F.Venegas Valencia, KhristianGaleano, Sandra P.Culebras, JaimeGuayasamin, Juan M.2025-02-042025-02-042024Franco-Mena D, De la Riva I, Vega-Yánez MA, Székely P, Amador L, Batallas D, Reyes-Puig JP, Cisneros-Heredia DF, Venegas-Valencia K, Galeano SP, Culebras J, Guayasamin JM. 2024. Simplifying the Centrolene buckleyi complex (Amphibia: Anura: Centrolenidae): a taxonomic review and description of two new species. PeerJ 12:e17712 https://doi.org/10.7717/peerj.177122167-835910.7717/peerj.17712https://hdl.handle.net/20.500.14352/117791This work was supported by Inédita Program from the Ecuadorian Science Agency SENESCYT (Respuestas a la Crisis de Biodiversidad: La Descripción de Especies como Herramienta de Conservación; INEDITA PIC-20-INE-USFQ-001), USFQ (Grants HUBI 5466, 17857, 16871). Ignacio De la Riva was funded by project PGC2018-097421-B-I00 (PI: I. De la Riva), funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”, by the “European Union”. The work of Paul Székely in Abra de Zamora was funded by Critical Ecosystem Partnership Fund through the “Amphibian Conservation in the Abra de Zamora Key Biodiversity Area of Ecuador” project (CEPF-108984), Rainforest Trust and Naturaleza y Cultura Internacional through the “Investigación científica, para la protección y monitoreo de especies de anfibios en el Área Clave de Biodiversidad Abra de Zamora” project (PROY_INV_BA_2022_3502 PY3502) and by Universidad Técnica Particular de Loja through the “Fortalecimiento a la gestión del Abra de Zamora, un área clave para la conservación de la biodiversidad y los servicios ambientales de los Andes sur de Ecuador” project (PROY_INV_BA_2022_3573). The work of Jaime Culebras was funded by Centro Jambatu, BBC Studios NHFP and Fundación Cóndor Andino.Centrolenidae is a Neotropical family widely distributed in Central and South America, with its species richness concentrated in the tropical Andes. Several taxonomic problems have been identified within this family, mostly related to species with broad geographic distributions. In this study, we assessed and redefined the species boundaries of the Centrolene buckleyi species complex, and formally described two new species from the Andes of Ecuador. These new taxa are recognized by a combination of morphometric, osteological, acoustic, and genetic data. Following IUCN criteria, we propose that the two new species should to be considered as Endangered (EN), mainly because of their small distributions and habitat loss. The C. buckleyi complex provides insights into the biogeography of closely related Andean species. As in other glassfrogs, speciation in Centrolene seems to be mediated by the linearity of the Andes, where gene flow can be restricted by topography and, also, local extinctions.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.7717/peerj.17712https://peerj.com/articles/17712/open access574.9597.851AndesAnuraBiogeographyGlassfrogsNew speciesSystematicsEcología (Biología)Zoología2505.01 Biogeografía2401.06 Ecología Animal2401.16 Herpetología