Reche Gallardo, Pedro AntonioKeskin, Derin BHussey, Rebecca EAncuta, PetronelaGabuzda, DanaReinherz, Ellis L2023-06-202023-06-2020061476-943310.1186/1476-9433-5-1https://hdl.handle.net/20.500.14352/50380Cytotoxic T lymphocytes (CTL) protect against viruses including HIV-1. To avoid viral escape mutants that thwart immunity, we chose 25 CTL epitopes defined in the context of natural infection with functional and/or structural constraints that maintain sequence conservation. By combining HLA binding predictions with knowledge concerning HLA allele frequencies, a metric estimating population protection coverage (PPC) was computed and epitope pools assembled. Strikingly, only a minority of immunocompetent HIV-1 infected individuals responds to pools with PPC >95%. In contrast, virus-naive individuals uniformly expand IFNgamma producing cells and mount anti-HIV-1 cytolytic activity. This disparity suggests a vaccine design paradigm shift from infected to normal subjects.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttp://www.medimmunol.com/content/5/1/1open access612.01757:004InmunologíaMicrobiología (Biología)Biología molecular (Biología)Bioinformática2412 Inmunología2414 Microbiología2415 Biología Molecular