Truchado Martín, Daniel AlejandroJuárez-Molina, MaríaRincón, SaraZurita, LucíaTomé-Amat, JaimeLorz López, María CorinaPonz, Fernando2024-04-192024-04-192024-04-131661-659610.3390/ijms25084327https://hdl.handle.net/20.500.14352/103238Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP–cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article1422-0067https://doi.org/10.3390/ijms25084327open access578577.2616-006.04Turnip mosaic virusVLPProtein AZ domainCetuximabSquamous cell carcinomaViral nanoparticlesMicrobiología (Biología)BiotecnologíaOncología3201.01 Oncología2414 Microbiología