Muñoz Noval, ÁlvaroSanchez Vaquero, VanessaPunzon Quijorna, EstherTorres Costa, VicenteGallach Perez, DarioGonzalez Mendez, LauraMontero, IsabelMartin Palma, Raul J.Climent Font, AurelioGarcia Ruiz, Josefa P2025-01-302025-01-302012-01-06Noval AM, Vaquero VS, Quijorna EP, Costa VT, P erez DG, M endez LG, Montero I, Palma RJM, Font AC, Ruiz JPG, Silv an MM. 2012. Aging of porous silicon in physiological conditions: Cell adhesion modes on scaled 1D micropatterns. J Biomed Mater Res Part A 2012:100A:1615–16221549-329610.1002/jbm.a.34108https://hdl.handle.net/20.500.14352/117079The surface properties of porous silicon (PSi) evolve rapidly in phosphate-buffered saline. X-ray photoelectron spectra indicate the formation of a SiOH and CO enriched surface, which becomes increasingly hydrophilic with aging time. Multiscale stripe micropatterns of Si and PSi have been fabricated by means of a high-energy ion-beam irradiation process. These micropatterns have been aged in physiological conditions and used to analyze human mesenchymal stem cell (hMSC) adhesion. The actin cytoskeleton of hMSCs orients following the uniaxial micropatterns. In the wider Si stripes, hMSCs are dominantly located on Si areas. However, for reduced Si widths, adhesion is avoided on PSi by a split assembly of the actin cytoskeleton on two parallel Si areas. These results confirm that nanostructured SiOH/CO-rich surfaces with hydrophilic character are specially adapted for the creation of cell adhesion surface contrasts.engjournal articlehttps//doi.org/10.1002/jbm.a.34108https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.a.34108restricted access620.1615.46Porous siliconPhysiological agingIon-beam modificationMesenchymal stem cellsCell guidesFísica de materiales3312 Tecnología de Materiales