Fernández Albarral, José AntonioHoz Montañana, María Rosa deMatamoros, José A.Chen, LeijingLópez Cuenca, InésGarcía Martín, Elena SalobrarSánchez Puebla, LídiaRamirez Sebastian, Jose ManuelTriviño Casado, AlbertoSalazar Corral, Juan JoséRamírez Sebastián, Ana Isabel2023-06-222023-06-222022-04-192227-905910.3390/biomedicines10050939https://hdl.handle.net/20.500.14352/71693Received: 17 March 2022 / Revised: 15 April 2022 / Accepted: 16 April 2022 / Published: 19 April 2022 This article belongs to the Special Issue The Contribution of Astrocytes to Human Neurodegenerative Diseases.Macroglia (astrocytes and Müller glia) may play an important role in the pathogenesis of glaucoma. In a glaucoma mouse model, we studied the effects of unilateral laser-induced ocular hypertension (OHT) on macroglia in OHT and contralateral eyes at different time points after laser treatment (1, 3, 5, 8 and 15 days) using anti-GFAP and anti-MHC-II, analyzing the morphological changes, GFAP-labelled retinal area (GFAP-PA), and GFAP and MHC-II immunoreactivity intensities ((GFAP-IRI and MHC-II-IRI)). In OHT and contralateral eyes, with respect to naïve eyes, at all the time points, we found the following: (i) astrocytes with thicker somas and more secondary processes, mainly in the intermediate (IR) and peripheral retina (PR); (ii) astrocytes with low GFAP-IRI and only primary processes near the optic disc (OD); (iii) an increase in total GFAP-RA, which was higher at 3 and 5 days, except for at 15 days; (iv) an increase in GFAP-IRI in the IR and especially in the PR; (v) a decrease in GFAP-IRI near the OD, especially at 1 and 5 days; (vi) a significant increase in MHC-II-IRI, which was higher in the IR and PR; and (vii) the Müller glia were GFAP+ and MHC-II+. In conclusion, in this model of glaucoma, there is a bilateral macroglial activation maintained over time involved in the inflammatory glaucoma process.engAtribución 3.0 Españajournal articlehttps://doi.org/10.3390/biomedicines10050939https://www.mdpi.com/2227-9059/10/5/939open access617.7-007.681611.8.018.24616.12experimental glaucomamicemicrogliaastrocytesMüller cellretinaGFAPMHC-IIocular hypertensioncontralateral eyeOftalmologíaAnatomía ocular3201.09 Oftalmología