Romero Martínez, BeatrizAranaz Martín, AliciaJuan Ferré, Lucía DeÁlvarez Sánchez, JulioBezos Garrido, JavierMateos García, Ana IsabelGómez-Mampaso, EnriqueDomínguez Rodríguez, Lucas José2024-01-172024-01-172006Romero, B., Aranaz, A., de Juan, L., Alvarez, J., Bezos, J., Mateos, A., Gómez-Mampaso, E., & Domínguez, L. (2006). Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the same spoligotyping profile as isolates from animals. Journal of clinical microbiology, 44(9), 3405–3408. https://doi.org/10.1128/JCM.00730-060095-113710.1128/jcm.00730-06https://hdl.handle.net/20.500.14352/935012006, American Society for Microbiology. All Rights Reserved. A. Aranaz has a fellowship from the Ramon y Cajal Programme (Spanish Ministry of Science and Technology/U.C.M.). This research was funded by project AGL 2001-2029 of the Spanish Ministry of Science and Technology and by the Spanish Ministry of Agriculture, Fisheries, and Food.PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for those spoligotypes that have a low frequency.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1098-660Xhttps://doi.org/10.1128/JCM.00730-06open access579.62Microbiología (Veterinaria)3109.05 Microbiología