Hidalgo Vico, SusanaPrieto Prieto, Antonio DanielAlonso Monge, Rebeca María Del MarRomán González, ElviraPla Alonso, Jesús2023-06-172023-06-172021-06-24Hidalgo Vico, S., Prieto Prieto, A. D., Alonso Monge, R. M. M. et al. «The Glyoxylate Cycle Is Involved in White-Opaque Switching in Candida Albicans». Journal of Fungi, vol. 7, n.o 7, junio de 2021, p. 502. DOI.org (Crossref), https://doi.org/10.3390/jof7070502.2309-608X10.3390/jof7070502https://hdl.handle.net/20.500.14352/7092Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans. The master regulator of the white-opaque transition WOR1 has been implicated in the adaptation to this commensal status. A proteomic analysis of cells overexpressing this transcription factor (WOR1OE) suggested an altered metabolism of carbon sources and a phenotypic analysis confirmed this alteration. The WOR1OE cells are deficient in using trehalose and xylose and are unable to use 2C sources, which is consistent with a reduction in the amount of Icl1, the isocitrate lyase enzyme. The icl1∆/∆ mutants overexpressing WOR1 are deficient in the production of phloxine B positive cells, a main characteristic of opaque cells, a phenotype also observed in mating type hemizygous mtla1∆ icl1∆/∆ cells, suggesting the involvement of Icl1 in the adaptation to the commensal state. In fact, icl1∆/∆ cells have reduced fitness in mouse gastrointestinal tract as compared with essentially isogenic heterozygous ICL1/icl1∆, but overproduction of WOR1 in an icl1∆/∆ mutant does not restore fitness. These results implicate the glyoxylate shunt in the adaptation to commensalism of C. albicans by mechanisms that are partially independent of WOR1.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://doi.org/10.3390/jof7070502https://www.mdpi.com/journal/jofopen access579CommensalismGlyoxylate cycleWo switchingFungal pathogenesisEpigeneticsOpaque cellsOxidative stressMicrobiología (Farmacia)3302.03 Microbiología Industrial