Urzainqui, AnaMartínez del Hoyo, GloriaLamana Domínguez, AmaliaFuente, Hortensia de laBarreiro, OlgaOlazabal, IsabelPilar MartinWild, MartinVestweber, DietmarGonzález-Amaro, RobertoSánchez-Madrid, Francisco2024-01-192024-01-192007Ana Urzainqui, Gloria Martínez del Hoyo, Amalia Lamana, Hortensia de la Fuente, Olga Barreiro, Isabel M. Olazabal, Pilar Martin, Martin K. Wild, Dietmar Vestweber, Roberto González-Amaro, Francisco Sánchez-Madrid; Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells1. J Immunol 1 December 2007; 179 (11): 7457–7465. https://doi.org/10.4049/jimmunol.179.11.74570022-176710.4049/jimmunol.179.11.7457https://hdl.handle.net/20.500.14352/94052Dendritic cells (DCs) have a key role in both the generation of the immune response and the induction of tolerance to self-Ags. In this work, the possible role of P-selectin glycoprotein ligand 1 (PSGL-1) on the tolerogenic activity of human DCs was explored. We found that the engagement of PSGL-1 by P-selectin on DCs induced the expression of c-Fos, IDO, IL-10, and TGF-β genes. Remarkably, stimulation of DCs through PSGL-1 with P-selectin enhanced their capability to generate CD4+CD25+Foxp3+ regulatory T cells, which expressed high levels of TGF-β1 mRNA, synthesized IL-10, and suppressed the proliferation of autologous CD4+CD25− T cells. Accordingly, we found that DCs from PSGL-1−/− mice expressed higher levels of MHC class II molecules, and exhibited an enhanced immunogenicity compared with wild-type mice. In addition, the percentage of CD4+CD25+Foxp3+ regulatory T cells in the thymus of PSGL-1-deficient animals was significantly reduced. Our data reveal an unexpected role of PSGL-1 on the tolerogenic function of DCs, and the regulation of the immune response.engjournal article1550-6606https://doi.org/10.4049/jimmunol.179.11.7457open access576.3577.27P-Selectin Glycoprotein Ligand 1Dendritic CellsBiología celular (Biología)Inmunología2407 Biología Celular2412 Inmunología