Gonzalez, ErnestoGarcía Montalvo, JorgeGallego Rojo, ElenaLadero Galán, MiguelBolívar Bolívar, Juan Manuel2026-01-212026-01-212026-01-05Gonzalez, Ernesto, et al. «Experimental Monitoring and Modeling of Oxygen Dynamics in Laccase-Catalyzed Phenolic Oxidation». Reaction Chemistry & Engineering, 2026, p. 10.1039.D5RE00450K. DOI.org (Crossref), https://doi.org/10.1039/D5RE00450K.2058-988310.1039/d5re00450khttps://hdl.handle.net/20.500.14352/130685Oxygen-dependent enzymes such as laccases play a central role in green oxidation processes, yet kinetic analyses often overlook the dynamic role of dissolved oxygen. In this study, we propose a useful methodology for the experimental monitoring and kinetic modeling of oxygen-consuming enzymatic reactions, demonstrated using the laccase-catalyzed oxidation of gallic acid and ferulic acid. The method integrates real-time monitoring of dissolved oxygen and substrate concentration with dynamic simulations accounting for oxygen mass transfer and bi-substrate kinetic models commonly used to describe laccase-catalyzed reactions. Experiments were conducted in both non-sparged and air-sparged systems to assess kinetic behavior under oxygen-limited and oxygen-sufficient conditions. The method yielded well-constrained kinetic constants for gallic acid and revealed a biphasic oxygen-uptake pattern for ferulic acid, arising from secondary oxidation of radical intermediates. Using Aspen Custom Modeler built-in non-linear regression and numerical integration algorithms, kinetic parameters were estimated by fitting them to complete time-course data, enabling accurate modeling of complex oxygen-dependent reactions. The proposed framework provides a practical tool for studying oxidase-catalyzed processes under conditions where dissolved-oxygen dynamics can be experimentally resolved, and supports more rational design of biocatalytic systems where oxygen availability governs the activity, selectivity and control of complex reaction networks.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articleDOI https://doi.org/10.1039/D5RE00450Khttps://pubs.rsc.org/en/Content/ArticleLanding/2026/RE/D5RE00450Kembargoed access54LaccasePhenolic oxidationEnzyme kineticsOxygen monitoringDynamic simulationOxygen transferStoichiometric analysisRadical couplingIngeniería químicaBioquímica (Química)3302 Tecnología Bioquímica3303 Ingeniería y Tecnología Químicas